由于環(huán)境因素和細胞內的正常代謝過(guò)程造成的DNA損傷，每個(gè)細胞每天都會(huì )發(fā)生1,000到1,000,000個(gè)。雖然這些只占人類(lèi)基因組約60億個(gè)堿基中的一小部分，但如果關(guān)鍵基因損傷未及時(shí)修復，可能會(huì )阻礙細胞的正常生理功能，進(jìn)而增加癌變可能。彗星實(shí)驗，或稱(chēng)單細胞凝膠電泳（Single cell gel electrophoresis，SCGE），是一種測量單個(gè)細胞DNA損傷的常用技術(shù)。其原理很簡(jiǎn)單，即在電泳場(chǎng)中，將受損細胞DNA（包含片段和鏈斷裂）與完整的DNA分離，通過(guò)顯微鏡可觀(guān)察到損傷細胞呈現出典型的彗星狀尾巴，然后通過(guò)測量計算彗尾大小對比出細胞DNA損傷的程度。因為彗星實(shí)驗的特點(diǎn)，該方法幾乎被用來(lái)評估任何類(lèi)型的真核細胞的 DNA 修復能力，包括雙、單鏈斷裂的不同的 DNA 損傷情況。是一種能快速、大通量檢測真核細胞DNA損傷進(jìn)而判別遺傳毒性的技術(shù)。

彗星實(shí)驗結果圖

彗星實(shí)驗原理雖簡(jiǎn)單，但操作繁瑣，需要豐富的實(shí)驗經(jīng)驗和技巧，尤其常常出現的“脫膠”問(wèn)題，困擾了許多科研人員。除此之外，有時(shí)為了跑出完美的“彗星”圖案放在paper里，還需要重復做許多次實(shí)驗，費時(shí)費力。為了解決上述問(wèn)題，我們推薦CellBiolabs的OxiSelectTMComet Assay Kit即彗星實(shí)驗試劑盒來(lái)檢測細胞的DNA損傷。該試劑盒不僅能讓彗星實(shí)驗化繁為簡(jiǎn)，還有兩種不同規格（3孔和96孔）的細胞電泳凝膠板供選擇，讓少量樣本和大量樣本的DNA損傷檢測通通輕松hold住。用該試劑盒做彗星實(shí)驗流程如下圖。

除了操作簡(jiǎn)便，OxiSelectTMComet Assay Kit還有以下優(yōu)點(diǎn)：

1)      適用于各種DNA損傷檢測，是一款非常好用的DNA損傷檢測篩選工具；

2)      試劑盒中的載玻片經(jīng)過(guò)特殊處理以粘附低熔點(diǎn)瓊脂糖，避免“脫膠”問(wèn)題出現；

3)      采用特殊的DNA熒光染料，能有效降低背景干擾，更加方便讀取實(shí)驗結果。

彗星實(shí)驗試劑盒信息：

為了滿(mǎn)足客戶(hù)更多樣的實(shí)驗需求，彗星實(shí)驗試劑盒內特殊處理電泳載玻片還可以單獨購買(mǎi)，詳情如下：

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